Closing the Cell Cycle Circle in Yeast: G2 Cyclin Proteolysis Initiated 2025

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During the second gap phase (G2), the cell prepares for the mitotic division. In mitosis, divided into prophase, prometaphase, metaphase, anaphase, telophase, and cytokinesis, the cell divides by segregating the chromosomes into two separate daughter cells.
During the G2 phase, many of the organelles and molecules required for cell division are produced. When the events of the G2 phase are completed, the cell is ready to enter the M phase and begin the process of cell division. The division of the cytoplasm itself. Cytokinesis usually occurs at the same time as telophase.
The cyclin A/CDK2 complex terminates the S phase by phosphorylating CDC6 and E2F1, and drives the cell-cycle transition from S phase to G2 phase, and subsequently activates CDK1 by cyclin A leading the cells to enter the transition to M phase. Upon mitosis, CDK1 activity is maintained by the complex cyclin B/CDK1.
Two major cell cycle controls have been identified in yeast; one acting in the G1 phase of the cell cycle results in commitment to the mitotic cycle and the second acting in G2 leads to the initiation of mitosis.
The G2 checkpoint is a critical control point in the cell cycle that occurs at the end of the G2 phase, just before the cell enters the mitotic phase and begins cell division. The G2 checkpoint ensures that the cell has completed DNA replication and that the replicated DNA is undamaged before entering mitosis [32].
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Proteolysis therefore drives cell cycle progression not only by regulating CDK activity, but by directly influencing chromosome and spindle dynamics. The periodicity of DNA replication and mitosis in eukaryotes contrasts with the continuous nature of most metabolic reactions that produce cellular growth.

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