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DNA sequencing refers to the general laboratory technique for determining the exact sequence of nucleotides, or bases, in a DNA molecule. The sequence of the bases (often referred to by the first letters of their chemical names: A, T, C, and G) encodes the biological information that cells use to develop and operate.
To study the exact order (or sequence) of someones DNA, researchers follow three major steps: (1) purify and copy the DNA; (2) read the sequence; and (3) compare to other sequences.
Key Sequencing Methods DNA Sequencing. Analyze the entire genome, focus on regions of interest with whole-exome and targeted sequencing, or study DNA-protein interactions. RNA Sequencing. Methylation Sequencing. High-Throughput Sequencing. Long-Read Sequencing.
In DNA, the bases are bound to a sugar molecule called deoxyribose. Another molecular group, phosphate, is also attached to the base. The three different connected molecules (the base, sugar, and phosphate) form what is called a nucleotide. A string or sequence of nucleotides forms a DNA strand.
Third generation sequencing technologies offer the capability for single molecule real-time sequencing of longer reads, and detection of DNA modification without the aforementioned assay. PacBio SMRT technology and Oxford Nanopore can use unaltered DNA to detect methylation.
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DNA sequencing involves three main steps: 1) using PCR to amplify DNA fragments, 2) introducing dideoxynucleotides that halt DNA strand elongation, and 3) employing a computer to analyze the fluorescent labels on the DNA fragments to determine the sequence. Created by Ronald Sahyouni.
Long-read sequencing methods Single molecule real time (SMRT) sequencing. Nanopore DNA sequencing. Massively parallel signature sequencing (MPSS) Polony sequencing. 454 pyrosequencing. Illumina (Solexa) sequencing. Combinatorial probe anchor synthesis (cPAS) SOLiD sequencing.
Depiction of whole-genome, whole-exome, and targeted sequencing.

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