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The PHS Policy requires that euthanasia be conducted in a manner that is consistent with the professional guidance for relieving pain and suffering of animals found in the American Veterinary Medical Association (AVMA) Guidelines for the Euthanasia of Animals (PDF).
What are the 3Rs? The principles of the 3Rs (Replacement, Reduction and Refinement) were developed over 50 years ago providing a framework for performing more humane animal research.
Among the basic principles generally accepted in our culture, three are particularly relevant to the ethics of research using animals: respect for life, societal benefit and nonmaleficence.
The 3Rs: Replacement, Reduction and Refinement, formulated by William Russell and Rex Burch, have become synonymous with the measures to improve the welfare of animals used in research and are now used as an ethical framework for improving laboratory animal welfare throughout the world.
The legislation is underpinned by the three Rs the principles of replacement, reduction and refinement which ensures that animal research is carried out only where no practicable alternative exists and under conditions which minimise any suffering.
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The 3Rs alternatives refers to the replacement, reduction, and refinement of animals used in research, teaching, testing, and exhibition. Drs. William Russell and Rex Burch first described the 3Rs in 1959 in their book, The Principles of Humane Experimental Technique.
The PHS Policy, frequently referred to as the NIH Policy, requires each institution receiving PHS funds for research involving animals to submit detailed information regarding the institutions program for the care and use of animals (including farm animals, mice, and rats) to the Office of Laboratory Animal Welfare (
The PHS Policy IV. B. 3. requires that: The IACUC shall prepare reports of their semiannual program reviews and animal facility inspections and submit the reports to the Institutional Official (IO).

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