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Cold sterilization involves immersion of items in a sterilant solution, such as glutaraldehyde or alcohol, for a predetermined period of time.
Typical sterilization temperatures and times are 132C to 135C with 3 to 4 minutes exposure time for porous loads and instruments. Like other sterilization systems, the steam cycle is monitored by mechanical, chemical, and biological monitors.
Final answer: The most accurate statement concerning cold sterilization is option B: most cold-sterilized instruments should be used only for minor procedures. Cold sterilization refers to the process of sterilizing instruments using chemical disinfectants that do not require heat.
In order to pass sterilization validation acceptance criteria, sterile processes must have a one in a million probability of unit contamination (or less). Sterility acceptance criteria are also known as sterility assurance levels (SALs). Further, PNSU is a common abbreviation for the probability of a nonsterile unit.
Steam or dry heat are the preferred methods to sterilize surgical instruments. Other methods (e.g., chemical) may be used but must be documented in the IACUC approved protocol. Steam: 250F, 15 psi, for 30 minutes.
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All items to be sterilized should be arranged so all surfaces will be directly exposed to the sterilizing agent. Thus, loading procedures must allow for free circulation of steam (or another sterilant) around each item.
Instruments placed into sterilization solutions must be free of organic material including blood. All cold sterilization chemicals are ineffective in penetrating organic material. 6. Instruments for cold sterilization must be completely immersed in the solution.
Standard Precautions Hand hygiene. Use of personal protective equipment (PPE)(e.g., gloves, masks, eyewear). Cough etiquette. Sharps safety. Safe injection practices (i.e., aseptic technique for parenteral medications). Sterile instruments and devices. Clean and disinfected environmental surfaces.

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