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What is mouse survival surgery?

Any surgery conducted on animals that are expected to recover from anesthesia is considered survival surgery. Refer to the IACUC Anesthesia guidelines for more details about rodent anesthesia.

When suturing the skin of rats and mice what type of suture pattern is recommended?

Close the skin as a separate layer using simple interrupted suture pattern with monofilament non-absorbable suture such as nylon or absorbable suture (silk is not appropriate due to wicking and poor tensile strength). Tissue adhesive, staples, or wound clips may also be used.

When should sutures wound clips or staples be removed from a skin incision in animals?

Sutures, staples, or wound clips must be removed 7-14 days following surgery. If animals will be euthanized within 14 days following surgery, removal of sutures prior to euthanasia is not necessary. Any foreign substance left in the incision for a long period of time serves as a nidus of irritation and infection.

What are the proper techniques for making incisions into a rat?

Skin incision is to be made using sterile scalpel blade/instruments. When making your initial incision, attempt a smooth single cut. Multiple incision attempts with a scalpel blade can create a surface area for bacteria to grow. All suture material must be sterile and remain sterile throughout procedure.

What sutures are best for mice?

For mice, suture gauges 4-0, 5-0 or possibly 6-0 suture are generally appropriate. For rats, suture gauge 3-0, 4-0 or 5-0 are generally appropriate. Dermal / Skin sutures require the use of sterile monofilament type suture such as nylon or PDS. For other tissues, synthetics, i.e., Vicryl, Maxon or PDS, are preferred.

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