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Paraffin Embedded Tissue Request Form - UNMC

Paraffin Embedded Tissue Request Form - UNMC 2025

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The document is a Paraffin Embedded Tissue Request Form used by researchers to request tissue samples for scientific studies. It includes sections for the principal investigator's information, billing details, study contact, request specifics such as type of tissue and number of slides needed, and additional notes for pathologist review. The form also outlines procedures for rush orders and provides links for further information.

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What device is used for slicing paraffin-embedded blocks?

The instrument used to cut sections is the microtome. This has an advancing mechanism which moves the object, the paraffin block, for a predetermined distance until it is in contact with the cutting tool, the knife or blade.

What is the procedure of section cutting of a paraffin block?

Paraffin section cutting involves the use of extremely sharp knives or blades to cut very thin sections of tissue ready for mounting onto glass slides, prior to staining. Once the slides are stained, they will be examined microscopically.

How do you cut paraffin embedded tissue?

Clamp the paraffin-embedded specimen on a rotary microtome and place the blade in a blade holder. Turn the handwheel and section the specimen. Transfer the paraffin sectioned ribbon to the 38.5 C warm water bath. Pick up the sections onto the microscope slide from the water surface after unfolding.

What is paraffin embedded tissue?

Abstract. Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block.

What are paraffin embedded sections of tissue are cut?

Paraffin sectioning is the procedure of cutting thin slices of tissue that has been dehydrated and infiltrated with wax using specialized equipment. This tissue is then embedded in wax before being cut on a microtome.

How do you extract DNA from paraffin embedded tissue?

To extract DNA from paraffin-embedded samples, tissue cores or microdissected tissue are subjected to xylene treatment, which dissolves the paraffin from the tissue, and then rehydrated using a series of ethanol washes. Proteins and harmful enzymes such as nucleases are subsequently digested by proteinase K.

How do you prepare tissue for paraffin embedding?

Paraffin embedding protocol Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample). Move cassettes into 70% ethanol for 24 hrs. Turn on wax pots. Move cassettes into 75% ethanol for 30 minutes.

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