Paraffin Embedded Tissue Request Form - UNMC 2026

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  1. Click ‘Get Form’ to open the Paraffin Embedded Tissue Request Form in the editor.
  2. Begin by filling out the Principal Investigator section. Enter your name, department, phone number, and email address. Ensure all required fields are completed.
  3. In the Billing section, provide your request number and cost center or grant number. If applicable, include your fax number.
  4. Next, complete the Study Contact section. You can choose to use the same information as the Principal Investigator or fill it out separately.
  5. For Request Details, list all de-identified case IDs from the NECARES website and specify the type of tissue required. Indicate any special slide requests and whether a rush order is needed.
  6. If pathologist review is required, select 'Yes' and specify your needs. Fill out any additional study information if you are a first-time user.
  7. Finally, review all entries for accuracy before saving or emailing the form directly from our platform.

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Formalin-fixed paraffin-embedded tissue can be stored long term at room temperature whereas frozen tissue can only be stored for up to one year at -80 C. Frozen tissues can be affected by the formation of ice crystals, which can affect subcellular detail and impact IHC staining.
Paraffin-embedded tissue blocks are cut using a microtome (Figure 14). A microtome (from the Greek mikros, meaning small, and temnein, meaning to cut) is a sectioning instrument that cuts extremely thin slices of material, known as sections. After sectioning, the slices can be placed on a slide.
FFPE tissue may not be suitable for analysis native proteins, and generally is inferior for the types of genetic analysis, such as PCR, qPCR, or next generation DNA sequencing, that are becoming commonplace. Frozen tissue, on the other hand, preserves the DNA, RNA, and native proteins.
Excellent Tissue Preservation: Paraffin embedding helps maintain tissue structure for long-term storage, allowing for accurate and reliable analysis of tissue morphology. Improved Sectioning: Paraffin blocks are easy to cut into thin sections, which are essential for detailed microscopic examination.
To embed samples: Fill a metallic embedding mould with paraffin. With warm tweezers; transfer the constructs. from the cassette to the mould with paraffin and push the constructs (cross-section down) to the bottom of the mould. Let stiffen slightly on a cold plate so the sample stays at the bottom of the mould.

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A frozen section examination is a specific type of biopsy procedure that allows a surgeon to establish a rapid diagnosis of a suspicious mass during surgery. The technical name for this procedure is cryosection.
Paraffin sections are more physically stable and superior to frozen sections in maintaining tissue morphology with less damage. But due to the wax infiltration process, paraffin sections are not optimal for some staining processes.

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