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Weekly Flush the chamber with clean, non-chlorinated water or chamber cleaning solution. This is especially important if you routinely sterilize saline solutions. Inspect the door gasket for tearing, pitting or warping. Visually check for water and/or steam leaks.
Unloading Do not open the autoclave while the chamber is pressurized, released steam can cause severe burns. Verify that the temperature and duration of exposure were met. Wait until the autoclave has cooled prior to opening the door. Avoid standing directly in front of the autoclave door when it is opened after a run.
Each unit used to decontaminate must have an autoclave log where the operator records the date, name, cycle time, and monthly biological indicator results.
They work with a combination of steam, pressure and time. Autoclaves operate at high temperature and pressure in order to kill microorganisms and spores.
Autoclaves are commonly used in healthcare settings to sterilize medical devices. The items to be sterilized are placed inside a pressure vessel, commonly referred to as the chamber. Three factors are critical to ensuring successful steam sterilization in an autoclave: time, temperature and steam quality.
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For the autoclave process to be effective in achieving sterilization, sufficient temperature, time and direct steam contact are essential.
Autoclave recordkeeping is an essential component of sterilization best practices. Industry standards stipulate that time, temperature, and pressure must be recorded for every cycle that is run, and those records must be maintained for three years.
Completed autoclave use logs must be retained by laboratory staff for 3 years. Autoclave record keeping will be confirmed during yearly laboratory safety surveys.

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