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How do you calculate calibration of a pipette?

Finally, calculate accuracy by using the equation A = 100 x Vavg/V0, where A is the accuracy of the pipette, Vavg is the average calculated volume and V0 is the theoretical volume you tried to dispense. If the accuracy value lies in the 99-101% range, the pipette is considered normal and calibrated.

What is the Z value of water?

shown in Table 4, the factor Z variation in distilled water due to water temperature is approximately 2.0 10 3 mL/g or 2.0 10 -4 mL/(g C) between 15 C and 25 C.

What is the Z factor in pipette calibration?

The calibration of pipette is carried out by gravimetric method. When determining the volume of water, the accuracy of measurements is effected by ambient temperature, atmospheric pressure and relative humidity. These factors are usually combined to give the Z factor, used in calculation of volume of water.

How do you calibrate a pipette step by step?

2:31 3:50 Pipette Calibration - YouTube YouTube Start of suggested clip End of suggested clip The most critical factors affecting precision are the calibration. Environment repeatable proceduresMoreThe most critical factors affecting precision are the calibration. Environment repeatable procedures and the personal skills of the calibrator.

What are the tolerance limits for pipette calibration?

These limits are based on a simple guideline: Two percent of full scale at all volume settings. For example, the systematic error for a 100 L variable-volume pipette is 2.0 L (2%) at the 100 L setting, and 2.0 L (4%) at the 50 L setting.

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What is the Z factor formula for pipette calibration?

The formula for calculating the volume dispensed by the pipette is V = w * Z where w is the weight of the water, Z is the conversion factor based on the density of the water, and V is the calculated volume of how much water was dispensed.

What is the standard for pipette calibration?

There are two primary types of ISO pipette calibration, which are known as ISO 8655 and ISO 17025. Pipettes are an essential piece of lab equipment that every laboratory needs. We expect pipettes to accurately dispense liquid but after a while, pipettes will need calibration.

What is the calibration method of a pipette?

The calibration of pipette is carried out by gravimetric method. When determining the volume of water, the accuracy of measurements is effected by ambient temperature, atmospheric pressure and relative humidity. These factors are usually combined to give the Z factor, used in calculation of volume of water.

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