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An alternative two-step testing method reduces the number of clinic visits from 4 to 3. The first TST is not read 48-72 hours after placement and is read at day 7. Most positive TSTs will remain positive for 7 days. Those that disappear or decrease in size by day 7 will be boosted to positive with the second TST.
Administering the TST. The Mantoux tuberculin skin test (TST) is performed by placing an intradermal injection of 0.1 ml of purified protein derivative (PPD) containing 5 tuberculin units (TU) into the volar surface of the forearm.
A two-step test is required. If the TST was done in the past 12 months, only a one-step test is required. If the result is positive, refer to *Person with a positive TST. If both tests are negative, only a one-step test is required.
If the Mantoux tuberculin skin test (TST) is used to test health care personnel upon hire (preplacement), two-step testing should be used. This is because some people with latent TB infection have a negative reaction when tested years after being infected. The first TST may stimulate or boost a reaction.
A 2 step is defined as two TST's done within 1month of each other. 2. What is the procedure for 2-step TB skin test? Both step 1 and step 2 of the 2 step TB skin test must be completed within 28 days.
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Most positive TSTs will remain positive for 7 days. Those that disappear or decrease in size by day 7 will be boosted to positive with the second TST.
How Often Can TSTs Be Repeated? In general, there is no risk associated with repeated tuberculin skin test placements. If a person does not return within 48-72 hours for a tuberculin skin test reading, a second test can be placed as soon as possible.
There are two kinds of tests used to detect TB bacteria in the body: the TB skin test (TST) and TB blood tests. A positive TB skin test or TB blood test only tells that a person has been infected with TB bacteria. It does not tell whether the person has latent TB infection (LTBI) or has progressed to TB disease.
to be repeated? If you have a negative skin test, you need a repeat test at least once every four years. If you have a documented positive skin test, you must have an initial chest X-ray. After that, you still need to be screened every four years.
The TB skin test is performed by injecting a small amount of fluid (called tuberculin) into the skin on the lower part of the arm. A person given the tuberculin skin test must return within 48 to 72 hours to have a trained health care worker look for a reaction on the arm.

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