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At 100x magnification you will be able to see 2mm. At 400x magnification you will be able to see 0.45mm, or 450 microns. At 1000x magnification you will be able to see 0.180mm, or 180 microns.
Starting with a low magnification objective, such as 10x or 20x, the bacteria are located by adjusting the focus knobs. Slow and careful movements ensure that the bacteria come into clear focus. For more detailed observation, higher magnification objectives like 40x or 100x can be used.
So lets study more about these parts of the microscope and their uses. Structural Parts: Head, Arm, Base. Optical Parts: Eyepiece, Eyepiece tube, Nosepiece, Objective lenses, Adjustment knob, Stage, Stage clips, Aperture, Microscopic illuminator, Condenser, and Diaphragm.
The compound microscope typically has three or four magnifications - 40x, 100x, 400x, and sometimes 1000x. At 40x magnification you will be able to see 5mm. At 100x magnification you will be able to see 2mm. At 400x magnification you will be able to see 0.45mm, or 450 microns.
There is no limit to magnification, but at ~100x magnification you get a higher pixel density (for a typical camera) than the resolution of the microscope. For super-resolution microscopy or deconvolution, it might be worthwhile to push a little higher than 100x.
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In short, as magnification increases, the field of view decreases. When looking through a high power compound microscope it can be difficult to determine what you will see through the eyepieces at different magnifications.
High power objective lenses in microscopes typically have magnifications ranging from 40x to 100x. These lenses are used for detailed examination of specimens and provide high levels of magnification, allowing for close-up views of fine structures and cellular details.
Compound microscope Compound microscopes have two lenses: the second lens magnifies the image enlarged by the first lens. Modern compound microscopes can provide a magnification of 1,000 times.

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