Parasitology Manual - Department of Microbiology - Mount Sinai ... 2025

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Microbiology and parasitology handle the diagnosis and treatment of infectious diseases and the pathogens that cause them. The latest advances in this speciality arise from the need to respond to new situations.
The examination for parasites by microscopy in routine diagnostic laboratories is mostly requested on faecal samples. Other specimens received for detection of parasites include , sputum, liver aspirates, duodenal aspirates, bile, corneal scrapings, contact lens fluid, and tissue.
Most parasitology textbooks and laboratory manuals recommend the examination of at least three independently collected stool specimens to maximize the sensitivity of detection of intestinal ova and parasites (O P) (1, 2, 5).
The most common specimen submitted to the diagnostic laboratory is the stool specimen, and the most commonly performed procedure in parasitology is the ova and parasite examination, which is composed of three separate protocols: the direct wet mount, the concentration, and the permanent stained smear.
An ova and parasite test looks for intestinal parasites and their eggs (ova) by checking a sample of your stool (poop) under a microscope. The test is also called an OP. Intestinal parasites are tiny life forms that can live and reproduce in your digestive system.

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The study of microorganisms, or organisms that are typically too small to be seen clearly with the unaided eye, is the focus of the biology subfield known as microbiology. Bacteria, viruses, protozoa, many algae, and fungi are among these creatures.
Parasitology Specimen Collection Guidelines Specimen TypeCollectiona Device Eye: corneal scraping for Acanthamoeba spp. Direct inoculation of non-nutrient agar coated with bacterial overlay of PBS. Feces: Preserved Sterile, leakproof, wide-mouth container. Unpreserved Sterile, leakproof, wide-mouth container11 more rows

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