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activity 4 area of staying in this weamp;#39;re going to use a simple threshold to identify regions and quantify the percentage of the total image next thing weamp;#39;re going to do is work out the area that weamp;#39;ve highlighted as a proportion of the entire area and also just in in micron squared as well okay so weamp;#39;ll leave the threshold there if you havenamp;#39;t donamp;#39;t have this image up then youamp;#39;ll have to go through the previous activity to get the threshold and next thing weamp;#39;re going to do is go to analyze and set measurements to tell fiji what measurements we want to read out in this case letamp;#39;s read our area integrated density mean grey value and area fraction a really important button here is limit to threshold otherwise we donamp;#39;t click this itamp;#39;s going to read out the entire image so not just the thresholded area i would click okay thatamp;#39;s set the measurements weamp;#39;re going to read out and now we can g