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♪ Pregnancy tests, whether done at home or in a lab, detect a substance called HCG, which stands for human chorionic gonadotropin. This is found in the blood and urine of pregnant women, and is normally produced by reproductive tissues at very high levels in the first 3-4 months of pregnancy. HCG is a hormone, and one of its purposes is to signal other parts of the body to do the things needed to sustain pregnancy. It can be detected early on in a pregnancy, so it is a suitable surrogate for figuring out whether someone may be pregnant. But how does the pregnancy test kit work? Home pregnancy kits are a type of lateral flow device, which means that when you apply a liquid, in this case urine, it flows sideways across the absorbant pad where it interacts with different reagents. Some of these reagents are attached to specific locations on the pad, so they line up with the spots for the control reaction and the test reaction. The whole process depends on HCG connecting to or binding to
