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we have imaged the tissue now we will remove the cover slip remove the coverslip immediately after Imaging is complete first dispense 800 milliliters of millicue water into a beaker immerse the slides sideways or in a horizontal orientation in the beaker containing 800 milliliters of water with the coverslip surface fully sideways hold the slide in the water until the cover slip slowly separates away from the slide to avoid damaging the tissue sections and capture areas or causing tissue Detachment do not move the slide up and down Shake forcibly or manually move the coverslip gently immerse the slide 30 times in the water to ensure that all mounting medium is removed wipe the back of the slide with a laboratory wipe place the slide on a flat clean non-absorbent work surface and air dry incubate the slide on the low profile thermal cycler adapter with the thermocycler lid open for 3 minutes at 37 degrees Celsius to dry the slide proceed immediately to de-cross linking or store
