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gene therapies use viral vectors including lentivirus herpes virus and adenovirus to deliver dna into cells with missing or incorrect dna adeno-associated viral vectors or aav are the leading tool and consist of a capsid or shell surrounding the dna manufacturing can produce different populations of capsids full containing a complete copy of the dna or empty containing no dna or an incomplete copy for safety and efficacy of the gene therapy maximizing the number of full capsids is critical downstream purification steps can help separate the empty and full capsids with the goal of up to greater than 95 full capsids analytical methods assess the effectiveness of the purification process including quantifying the ratio of empty to full capsids some analytical methods like ion exchange chromatography elisa and ddpcr assays are relatively straightforward but the inability to accurately quantify empty capsids can cause an under or over prediction of full capsids electron microscopy-based met