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hi guys this is Dan croucher field application specialist with photo metrics and today Im going to talk about light sheet microscopy so firstly lets set out what the challenges are of conventional light microscopy so we know what we need from a microscope life is full of dynamic processes so in order to visualize them we need to image live cells or organisms so the first thing we want to do is avoid photobleaching of photo damage to live samples so we can be sure that the processes were following occur in healthy living cells we also want to capture these dynamic processes in three dimensions because things dont happen just in a single focal plane samples arent all the same size so we need to be able to image varied sample sizes as well as image them from various viewing positions finally we want to minimize out of focus for essence for good optical sectioning the problem is that conventional microscopy on coverslips isnt so compatible with live samples theres no way we can squ