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In this video tutorial, the focus is on the termination of transcription in prokaryotes, specifically in E. coli. Termination occurs in two ways: rho-independent and rho-dependent. Rho-independent termination does not involve the rho protein, while rho-dependent termination does. In rho-independent termination, a modified structure of the RNA transcript, particularly a hairpin loop, facilitates the release of the RNA transcript from the RNA polymerase complex, thereby ending transcription. Conversely, in rho-dependent termination, the helicase protein (rho protein) is involved in terminating transcription. The video highlights the significance of the hairpin loop structure in rho-independent termination.