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[Music] [Laughter] [Music] so orthogonal views these are really useful if you want to inspect some of the data in a 3d model ive got here a two-channel data set its a blood vessel thats been perfused under pressure and then fixed see its got a nice shape to it and if we wanted to inspect this data um using xz or yz sectioning then we would use the orthogonal planes we would go into the image menu stacks orthogonal views and what we immediately see here is that this data set is far too thin the properties are not set properly ill go to my image properties which is here and youll see that there is one channel 64 slices this is the pixel size 0.3 by 0.3 and x and y but i know that this z step in this case was actually one micron so its important to get the depth correct click ok and then stacks orthogonal views its much better looking okay i think its just popped off the screen now though let me just move this back so heres our xz image heres our yz so imagine if this is if th
