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for urine collection using a vacutainer you will need clean gloves your analysis tube culture and sensitivity tube vacutainer and a clean pad or paper towel give the vacutainer to the patient and instruct on how to collect a clean midstream urine sample next apply clean gloves receive the urine sample as soon as possible from the patient and place upright on a clean paper towel then peel back the yellow warning label covering the needle transfer device if collecting a ua and cns collect the cns before the ua place the tube stopper directly over the open transfer device and push firmly down for the needle to pierce the stopper negative pressure will allow the urine to flow into your collection tube if there is a preservative invert the tube eight to ten times to mix repeat with your analysis tube holding tube in position until filled label specimen tubes with patient labels as per hospital policy then apply the yellow warning label back over transfer device and dispose of in a sharps bi