Take out sample in PAP

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Aug 6th, 2022
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How to take out sample in PAP

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hello and welcome to the channel love of status and gynecology in this video iamp;#39;ll explain about the technique of a pap smear and the liquid-based cytology for the cervical screening so the things that you will require for a pap smear or a liquid-based cytology a pair of gloves some clean glass slides this is known as the iron spatula the cyto brush we have the cuscoamp;#39;s by well speculum and for the liquid-based cytology we have this one known as the cytobroom and a liquid-based cytology while so beginning with the processor so first you need to perform an adequate hand hygiene and after that done a sterile pair of gloves as per your size and then finally you will introduce the cuscoamp;#39;s speculum see cusco speculum is for the retraction of anterior and the posterior vaginal wall right but we donamp;#39;t introduce it as such itamp;#39;s very uh very traumatic for the patient so basically you rotate it to a 90 degree like that and then you insert it right through an

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an adequate sampling from the cervix using a broom-like device. Insert the central bristles of the broom into the endo- cervical canal deep enough to allow the shorter bristles to fully contact the ectocervix. Push gently, and rotate the broom in a clockwise direction five times.
Pap test. During a Pap test, a tool called a speculum holds the vaginal walls apart. A sample of cells from the cervix is collected using a soft brush and a flat scraping device called a spatula (1 and 2). The cells are placed in a bottle that contains a solution to preserve them (3).
PLEASE NOTE: The specimen does NOT need to be refrigerated or packed with ice.
We may want a urine specimen when you come for your appointment, so do not empty your bladder just prior to coming. Best time of month for test: 11-19th day of cycle count day one as the beginning of the menstrual flow (cannot do test during menses).
Your healthcare provider will use a small brush, swab or spatula to gently remove cells from the cervix and back of the vagina. They will place the cells in a vial of liquid or smear the cells on a glass microscope slide. If you need an HPV test, your provider will take a sample of cells for this test as well.
Women who are 21 to 29 should have a Pap test alone every 3 years. HPV testing alone can be considered for women who are 25 to 29, but Pap tests are preferred. Women who are 30 to 65 have three options for testing. They can have a Pap test and an HPV test (co-testing) every 5 years.
Rotate a spatula 360 once to obtain a single sample. Smear the sample onto the labeled slide. Fix the sample immediately (before it is air-dried) using a cytology spray fixative. Hold the fixative 15-20 cm (6 to 8 inches) away from the slide and evenly spray the slide by depressing the plunger 2 or 3 times.
Obtain an adequate sampling from the endocervix using an endocervical brush device. Insert the brush into the endocervical canal until only the bottom-most fibers are exposed. Slowly rotate or turn in one direction. DO NOT OVER ROTATE.

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