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this is a defaulting column short and stout small things stay in well bigger things flow out the reason why this happens is they take a shorter route so put your sample in and swap those buffers out so these are desalting columns um or buffer exchange columns and so these small ones i typically use them to remove radioactive atp from rna after a labeling reaction and there are also um ones that you can use for like proteins for desalting um removing various salts and other small molecules that might interfere with downstream steps things like tris or edta that might be incompatible with future things um you can and exchange basically exchange the buffer so that youre putting it in a liquid that you want it to be in and so it is an alternative when youre doing it for proteins and stuff it is an alternative to dialysis well talk about some of the pros and cons of different methods but the same principles are at play in each of these different things i remember how shocked i was when