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collecting a sample at a weightable site weighed as far into the water as is safe and comfortable to get to flowing water try to avoid collecting water from right next to the bank unless that is the only safe way for you to collect your sample the goal is to collect a sample from mid depth not the surface water or in a way that disturbs and collects sediment from the bottom once you weighed in wait for any sediment that you may have Disturbed to settle and then sample Upstream from your body collect the sample in a u shape putting the bottle straight down turning it under water to be parallel with the stream bed and allowing the bottle to fill you may push it gently Upstream if necessary then lift it straight up out of the water use a gentle flick to remove excess water from the bottle carefully secure the cap making sure that it is properly aligned and fully tightened to ensure that it does not leak again fill the bottle using a u-shape straight down forward and then back up flick to