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have you ever been setting up your qPCR experiment and been stuck trying to decide which reverse transcription or RT method to use youamp;#39;re not alone weamp;#39;ve received quite a few questions about RT methods so I thought it might be a good idea to learn about what methods are out there and why choosing the right method matters so letamp;#39;s get technical and look at our lab there are two primary approaches to reverse transcription for qPCR known informally as two-step or one stepamp;#39; methods in the two-step approach the first step is reversed transcription of the RNA to generate first strand cDNA or complimentary DNA at this point cDNA can be stored or moved to the second step in the second step a second strand cDNA is generated and real-time detection is performed the other method known as one-step is where the reverse transcription and amplification are performed together in the same tube pretty self-explanatory nomenclature right so how do you know which method to
