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antibiotic sensitivity testing to determine the antibiotic sensitivity of bacteria the following method is used so get sterile swab in a diluted bacterial culture and press it against the inner wall of the tube to remove excess fluid carefully swab the entire surface of the mule hinton agar plate ensuring that the swab overlaps each time to evenly distribute the inoculum turn the plate 120 degrees and swab again repeat a third time place the used swab in aswan bag then using the antibiotic disc dispenser stamp the disks over the swab surface incubate at 37 degrees overnight after overnight incubation examine your Miele Hinton plate if zones appear around the disks then the organism is sensitive to the antibiotic however if no zones appear around the disks then the organism is resistant to the antibiotic sensitivity patterns can also be measured and recorded as either sensitive or resistant by measuring the inhibition zone size and comparing the results against a reference table of anti