Strike out stain in PAP

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Aug 6th, 2022
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How to strike out stain in PAP

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hi everyone for todayamp;#39;s topic we will be discussing about the papa nicolao staining technique but before that i want you to know that papa nicolau stain or the pop staining method is under the big umbrella of diagnostic cytology under diagnostic cytology it also belongs under exfoliative cytology so letamp;#39;s differentiate first what diagnostic cytology and exfoliative cytology means diagnostic cytology class simply means microscopic examination of cells from different body sites for diagnostic purposes diagnostic cytology includes exfoliative abrasive and aspirational but for today letamp;#39;s focus on exfoliative cytology deals with the microscopic study of cells that have been disqualmated from epithelial surfaces exfoliated cells may be found in smears that have spontaneously been shed or physically removed from epithelial and mucous membranes such as the vagina bucal mucosa and from body fluids such as the sputum urine plural peritoneal or acetic fluid the gastric ju

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Protocol for Gills 1X and 2X Hematoxylin stain for Papanicolaou stains Fix Pap smears in 95% Ethanol: 15 minutes. Rinse gently in DI water: 30 seconds. Stain in hematoxylin solution, Gill 1X or Gill 2X: 1 to 3 minutes. Rinse gently in DI water: 1 minute. Bluing Reagent: 15 to 60 seconds.
The Papanicolaou stain is undoubtedly the most commonly used in cytology. It has three cytoplasmic dyes, orange G, eosin Y, and light blue. It can stain cells blue or blue-green (basophilia, cyanophilia), pink (acidophilia, eosinophilia), orange (orangeophilia), or indeterminate (gray-blue).
The speculum gives a clear view of the cervix and upper vagina. Cells are removed from the cervix with a brush or other sampling instrument. The cells usually are put into a special liquid and sent to a laboratory for testing: For a Pap test, the sample is examined to see if abnormal cells are present.
PAP bluing solution is a variant of Scotts solution and is used specifically in Papanicolaou staining (Pap staining) for cytological smears. It is used to bluish the hematoxylin in the stained nuclei to improve contrast and visibility, which facilitates the interpretation and analysis of cytology specimens.
Procedure: Remove cover slip by incubation in xylene for a few hours to several days (until the coverslip can be removed easily). Rehydrate slides in 100%, 85%, and 75% ethanol, each for 30 sec at room temperature. Wash slides in deionized or distilled water for 5 min at room temperature.
LEEP stands for Loop Electrosurgical Excision Procedure. Its a treatment that prevents cervical cancer. A small electrical wire loop is used to remove abnormal cells from your cervix. LEEP surgery may be performed after abnormal cells are found during a Pap test, colposcopy, or biopsy.
Wash smears in running water for 1 minute. NOTES: This step removes spray-fixative and the timing is for use with water soluble fixatives. Initial washing is important and must be thorough to expose the cell to staining. If slides have been fixed in 95% ethanol, timing may be shortened to a few seconds.
During a Pap test, a tool called a speculum holds the vaginal walls apart. A sample of cells from the cervix is collected using a soft brush and a flat scraping device called a spatula (1 and 2). The cells are placed in a bottle that contains a solution to preserve them (3).

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