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right so I wouldnamp;#39;t I like to do is try and make this better and to do that weamp;#39;re going to explore each of these different parameters here and see what effect that we get from changing them so the first thing that I do if the results are not particularly good and in this case I I donamp;#39;t think theyamp;#39;re particularly good thereamp;#39;s always a little bit of a judgment call as to when is Wendy honestly want to detect a nucleus weamp;#39;re not when is it sufficiently in focus or sufficiently Stian for you to actually want to detect it but there are some things that donamp;#39;t look very good to me like some apparent fragmentation there where one nucleus is counted multiple times as others where it may be arguably missing you baby you really should be detecting and whenever that happens the first parameter is right here the start thatamp;#39;s the one that I want to look at first and that is the detection image weamp;#39;ve two choices the hematoxylin o
