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this tutorial take through the steps for producing khaimah grams of live data here we have a hyper stack two channels acquired over time the extractor is moving up and diana filament first Iamp;#39;m going to separate out channels split channels and weamp;#39;re going to perform a comma gram on one channel at a time so select first channel draw a line region of interest and now we need to restore that selection edit selection restore selection on to the other channel and then go to analyze multi coma gram select a multi kinda gram option this then allows us to define the number of pixels that we want to analyze over in this case our images 30 pixels wide so we choose 29 and this gives us a comma Graham read out over time this can be saved as tiff file save this is k1 keep the process for the second channel analyze multi coma Graham all Tacoma Graham 29 pixel is one line width and now we can merge these two back together save this is Katie so letamp;#39;s merge the green at my channe
