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so having gone through the basics of a Hamp;amp;E stain and talked about the various features that we should be seen we can now start to look at some example slides and talk about some of the problems that can occur these particular slides Iamp;#39;ve selected from a set of slides that was submitted last semester and generally theyamp;#39;re all pretty good because theyamp;#39;ve been handed up for assessment but looking closely we can find some examples of things that have not gone perfectly and so we can discuss ways to try and correct that letamp;#39;s begin with a couple of really good slides so this first one here that weamp;#39;ve got on the screen is a pretty much perfect Hamp;amp;E of colon we can see thereamp;#39;s good nucleus staining there is not a lot of mucin staining so I guess itamp;#39;s been reasonably well differentiated but it doesnamp;#39;t look like itamp;#39;s been over differentiated because the nuclei still look very strong if we try and find