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hello and welcome to todays talk about next generation sequencing which is also known as massively parallel or just deep sequencing next-generation sequencing has completely revolutionized genome analysis with next-gen sequencing it is possible to sequence an entire human genome in just one day we start with the sample preparation once we have isolated our DNA we need to generate smaller fragments now all of these DNA fragments will get ligated to certain oligonucleotides there are two types here these include sequencing binding sites and also an additional complementary sequence which can then hybridize to the flow cell first our DNA double stranded fragment is deena traded so in theory the whole genome should be in fragments and all of the fragments have attached to different types of binding sites on each end all the DNA fragments are now applied on a ship the so called flow cell and as you can see here there are two types of complementary sequences the blue ones and the red ones i