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in this video lesson were going to how to chemically fix to a microscope slide using methanol we have a slide that weve prepared this is an air dried smear of the bacteria coming off of an agriculture and we learned how to do this preparation in a previous video lesson so we place our microscope slide with the air dried smear on our staining tray then were going to take a few drops of methanol and drop that over this mirror were then going to let that sit for two minutes now that two minutes have gone by all we do is pick up our slides were going to let the methanol drain off into the staining tray and then were going to put it back on the rack and we have to let it completely air dry at that point the slide will be ready for staining