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[Music] so welcome to another troubleshooting video this time with some pas stain slides so Ive been rummaging through some student collections and again these were actually from the five best exercise last year so its quite difficult finding some dips um some badly stained slides to be honest but theres a couple here that have cropped up and so we can use that as an example to discuss some of the problems that can occur now generally speaking the pas stain is a very straightforward stain and when people start to learn the stain probably the most common problem is just accidentally having the slides placed upside down and the reason why you often dont realize it hasnt worked until too late is because both of the reagents that youre using dont have a lot of color and effect theres potentially colorless so you you cant always tell straight away that theres an issue unlike something like hematoxylin where if the slide was accidentally upside down if you were standing on the sta