Slide stain in VIA

How to slide stain in VIA with no hassle

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Take these simple steps to slide stain in VIA

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How to Slide stain in VIA

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the funnest part of your CBC is making blood smears and its usually the bane of peoples existence but we are going to make it so you dont have any problems by the time this video is over first thing is you want to have a well-mixed unclouded EDTA tube obviously you want to mix it gently you dont want to be shaking it if youre concerned there may be clot in the sample which is obviously going to affect your numerical parameters most notably your platelets you can take an applicator stick and just run it through your sample and if there are any clots in there it will adhere to your applicator stick so if you dont have that you know you have a good sample the number one thing of making good smears is you want to make sure that you have bevel edge slides that is hugely important its almost impossible to make good blood smears without that as your tool and they can be used just for spreading if economy is an issue you want to start out with a well-filled PCV tube to get a decent siz

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Do you need stain for microscope slides?

Smear Preparation. Not only are most bacteria very small, they are also very clear and difficult to view under a microscope without first staining. You must firmly attach your bacteria to a glass slide before you can stain them.

Where do you place the stain in a glass slide?

Put a drop of stain on an outer edge of your cover slide. Place a piece of napkin or paper towel against the opposite side of your cover slip, right up against the edge. This will help draw the stain under the cover and across the specimen.

What liquid is used on microscope slides?

Wet Mount. In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. Place a sample on the slide. Using a pipette, place a drop of water on the specimen.

What is place on top of the slide sample and stain?

A small square or circle of thin glass called a coverslip is placed over the specimen. It prevents the slide from drying out when it's being examined. Iodine stain can be used to stain plant cells to make the internal structures more visible.

What is the procedure for staining a slide?

Simple Stain Procedure Place your carefully prepared fixed smear slides on the stain rack over the sink. ... Let the stain sit for 1-5 minutes. Using the clothespin, grab the long end of the slide, tilt the slide over the sink and rinse the stain off with a stream of water from the wash bottle.

What do you use to stain slides?

Microscope Slide Stains Iodine: Stains carbohydrates in plant and animal specimens brown or blue-black. ... Methylene blue: Stains acidic cell parts (like nucleus) blue. ... Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. ... Toluidene blue: Stains acidic cell parts (like nucleus) dark blue.

What stain is used for slides?

The Most Common Stains Used Haematoxylin and eosin combined make up a common stain most histology labs use first. No doubt you're familiar with at least haematoxylin, which is sometimes used alone. Combining this with eosin helps bring better definition to tissues and cells using a specific color chart.

How do you apply the stain to the slide?

Place some paper towel against the opposite edge of the cover slip - as close to the edge as possible. The paper towel will draw the stain underneath the cover slip. Wait until all of the stain has been pulled in between the cover slip and the slide. The stain should completely cover the specimen on the slide.

What are the correct steps in preparing a slide of bacteria for staining?

In order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide. This basically involves three steps----transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide.

Where do you place the stain in a glass slide?

Put a drop of stain on an outer edge of your cover slide. Place a piece of napkin or paper towel against the opposite side of your cover slip, right up against the edge. This will help draw the stain under the cover and across the specimen.

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Slide stain in VIA smoothly

How to slide stain in VIA with no hassle

Take these simple steps to slide stain in VIA

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How to Slide stain in VIA