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staining slides for AFB detection using the fast method requires the following materials fast army no stain fast decolorizer in quencher water or sterile water if possible to begin transfer your heat fix slides to staining rack quality control is very important for verification of your reagents and microscopy system therefore for quality control purposes include a control slide with each batch of stain slides if possible to apply the fast army no stain dispense eight to ten drops or enough stain to completely cover the smeared area allow the slide to sit for one minute at room temperature rinse each slide with water completely removing the stain use sterile water if possible be careful to not forcibly wash off and potentially remove the specimen gently shake off any excess water to apply the fast decolorizer quencher stain dispense eight to ten drops or enough stain to completely cover the smeared area allow the slide to sit for one minute at room temperature again rinse each slide wi