Slide stain in OSHEET

How to slide stain in OSHEET

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How to Slide stain in OSHEET

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so hi micro punter here I have a solution with the so called methylene blue according to Lafleur also known as the flood solution and in this video Im gonna show you how to use methylene blue to stain cells and specifically Im going to show you how to spay a stain the cheek cells Im also gonna take some cells from my teeth some bacteria that is Im gonna show you how you can stain your bacteria and at the very end Im going to put some drop of blood under the microscope and methylene blue is gonna stain the white blood cells but before I do that you have to be with me because Im going to now first tell you a little bit of background information some theory has to be when you buy yourself a set of stains and sometimes you will see that there are two bottles of methylene blue included one of them is called methylene blue just more saturated solution of methylene blue or concentrated methylene blue and then one is called methylene blue according to the flood or also known as basic me

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What can be used to stain structures when preparing a microscope slide?

Iodine stain can be used to stain plant cells to make the internal structures more visible. Most cells are colourless. Stains are used to add contrast. Certain stains are also used to stain specific cell structures or cell products.

What do you stain slides with?

Microscope Slide Stains Iodine: Stains carbohydrates in plant and animal specimens brown or blue-black. ... Methylene blue: Stains acidic cell parts (like nucleus) blue. ... Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. ... Toluidene blue: Stains acidic cell parts (like nucleus) dark blue.

How do you prepare a stain slide?

How to Stain Slides Prepare a wet mount or dry mount with a coverslip. Add a small drop of stain to an edge of the coverslip. Place the edge of a tissue or paper towel on the opposite edge of the coverslip. Capillary action will pull the dye across the slide to stain the specimen.

What is the procedure for staining a slide?

Simple Stain Procedure Place your carefully prepared fixed smear slides on the stain rack over the sink. ... Let the stain sit for 1-5 minutes. Using the clothespin, grab the long end of the slide, tilt the slide over the sink and rinse the stain off with a stream of water from the wash bottle.

What stain is used for slides?

The Most Common Stains Used Haematoxylin and eosin combined make up a common stain most histology labs use first. No doubt you're familiar with at least haematoxylin, which is sometimes used alone. Combining this with eosin helps bring better definition to tissues and cells using a specific color chart.

Where do you place the stain in a glass slide?

Put a drop of stain on an outer edge of your cover slide. Place a piece of napkin or paper towel against the opposite side of your cover slip, right up against the edge. This will help draw the stain under the cover and across the specimen.

What can I use to stain microscope slides?

There are several types of stains you can use at home including: food coloring, iodine, malachite green (ick fish cure), and methylene blue. You can purchase food coloring at a grocery store, iodine at a pharmacy, and the malachite green and methylene blue can be purchased at an aquarium store.

Do you need stain for microscope slides?

Smear Preparation. Not only are most bacteria very small, they are also very clear and difficult to view under a microscope without first staining. You must firmly attach your bacteria to a glass slide before you can stain them.

What liquid is used on microscope slides?

Wet Mount. In a wet mount, a drop of water is used to suspend the specimen between the slide and cover slip. Place a sample on the slide. Using a pipette, place a drop of water on the specimen.

What can you use to stain cells?

Methylene blue - stains animal cells to make nuclei more visible. Neutral/Toluylene red - stains nuclei red and may be used on living cells. Nile blue - stains nuclei blue and may be used on living cells.

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