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this demonstration is to show you how to focus on a prepared slide a prepared slide that you have stained right now Im going to show you how to focus on a simple stain when you look at a stained slide you should be able to see some coloration in this case I can see the purple dye on the slide turn on your microscope the scanning power is in place Im going to adjust the eyepiece to accommodate the distance between my eyes Im going to position the slide in between the stage arms and move the slide until the colored stain is right below the objective begin to take a look at the slide when using your scanning power youre going to use your coarse adjustment knob to get it in focus if your stain is very thick and dense you need to move to the edge of the smear so that you can see individual cells against a white background when looking at stain slides you want to make sure that your iris diaphragm is open and the background is nice and bright after I get the object in focus under scan I