Slide stain in NB

How to slide stain in NB with top efficiency

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How to Slide stain in NB

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Hi Im Megan today were going to stain a blood smear so that we can look at it under the microscope. Now here in this hospital we use the right stain and so the times that im going to give you for leaving the slide in each jar are consistent for what we use at this hospital - it may be a little bit different where you are. Its also important to make sure that your stain jars stay clean and that they stay free of debris. What we do here is we have a set of stain for our CBCs and cytologies, we have one for urine and we have one for fecals. And thats so that we dont cross contaminate and start to get really interesting results on our different cytologies and UAs so we make sure were not mixing things. We also empty the stain out of these jars, clean the jars and let them dry at least once a week so thats going to vary also depending on where you are and how often you use them but you can get things that grow in your stain that will show up on your blood films and its going to y

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How do you prepare a slide for staining?

How to Stain Slides Prepare a wet mount or dry mount with a coverslip. Add a small drop of stain to an edge of the coverslip. Place the edge of a tissue or paper towel on the opposite edge of the coverslip. Capillary action will pull the dye across the slide to stain the specimen.

What stain is used for slides?

The Most Common Stains Used Haematoxylin and eosin combined make up a common stain most histology labs use first. No doubt you're familiar with at least haematoxylin, which is sometimes used alone. Combining this with eosin helps bring better definition to tissues and cells using a specific color chart.

What stain is used for slides?

The Most Common Stains Used Haematoxylin and eosin combined make up a common stain most histology labs use first. No doubt you're familiar with at least haematoxylin, which is sometimes used alone. Combining this with eosin helps bring better definition to tissues and cells using a specific color chart.

What are the correct steps in preparing a slide of bacteria for staining?

In order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide. This basically involves three steps----transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide.

How do you properly stain a slide?

0:00 0:56 How to Stain a Slide - YouTube YouTube Start of suggested clip End of suggested clip We're going to put a drop on one side. And it wicks over just a little bit. But if we take the paperMoreWe're going to put a drop on one side. And it wicks over just a little bit. But if we take the paper towel and put it on the other side whoa. It basically sucks the stain across the slide.

What do you stain a microscope slide with?

Microscope Slide Stains Iodine: Stains carbohydrates in plant and animal specimens brown or blue-black. ... Methylene blue: Stains acidic cell parts (like nucleus) blue. ... Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. ... Toluidene blue: Stains acidic cell parts (like nucleus) dark blue.

Which stain is used for permanent slide?

Two different techniques are involved in the preparation of a permanent slide. Staining: In this method, safranin and haematoxylin are used.

What is the procedure for staining a slide?

Simple Stain Procedure Place your carefully prepared fixed smear slides on the stain rack over the sink. ... Let the stain sit for 1-5 minutes. Using the clothespin, grab the long end of the slide, tilt the slide over the sink and rinse the stain off with a stream of water from the wash bottle.

What can I use to stain microscope slides?

There are several types of stains you can use at home including: food coloring, iodine, malachite green (ick fish cure), and methylene blue. You can purchase food coloring at a grocery store, iodine at a pharmacy, and the malachite green and methylene blue can be purchased at an aquarium store.

How do you properly stain a slide?

0:02 0:56 How to Stain a Slide - YouTube YouTube Start of suggested clip End of suggested clip We're going to put a drop on one side. And it wicks over just a little bit. But if we take the paperMoreWe're going to put a drop on one side. And it wicks over just a little bit. But if we take the paper towel and put it on the other side whoa. It basically sucks the stain across the slide.

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Slide stain in NB smoothly