Slide stain in DWD

How to slide stain in DWD

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How to Slide stain in DWD

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in this video Im going to show you how to make a peripheral blood smear and for this youre going to need some slides you are also going to need some anticoagulated blood preferably EDTA and Im using a cat piercer to make the blood drop first thing you need to do is your cat piercer and then go ahead and get two slides out the first slide youre going to add a small drop of blood to and the second slide youre going to use place it on top and slowly bring it back until it makes contact with the drop and then rapidly push it forward and hopefully after doing this the smear will be about two-thirds way down the slide and youll get a nice feathered edge at the end there also you can make a slide thicker or thinner depending on the angle of the second slide now if you wanted to make it thicker you would just hold it at a higher angle and that makes the smear much thicker as you can see after I add the drop Im going to take the slide and hold it in a much larger angle and this

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What are stains for microscope slides?

Microscope Slide Stains Iodine: Stains carbohydrates in plant and animal specimens brown or blue-black. ... Methylene blue: Stains acidic cell parts (like nucleus) blue. ... Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. ... Toluidene blue: Stains acidic cell parts (like nucleus) dark blue.

What stain is used for slides?

The Most Common Stains Used Haematoxylin and eosin combined make up a common stain most histology labs use first. No doubt you're familiar with at least haematoxylin, which is sometimes used alone. Combining this with eosin helps bring better definition to tissues and cells using a specific color chart.

Where do you place the stain in a glass slide?

Put a drop of stain on an outer edge of your cover slide. Place a piece of napkin or paper towel against the opposite side of your cover slip, right up against the edge. This will help draw the stain under the cover and across the specimen.

Why do microbiologist stain slides?

This is because most microbes and cells lack colour and contrast. Laboratory staff and scientists use different mounting techniques, combined with dyes and microbiology stains to add contrast to specimens and make them easier to observe at a microscopic level and aid with identification.

What is the purpose of staining?

Staining is used to highlight important features of the tissue as well as to enhance the tissue contrast. Hematoxylin is a basic dye that is commonly used in this process and stains the nuclei giving it a bluish color while eosin (another stain dye used in histology) stains the cell's nucleus giving it a pinkish stain.

Which stain is used to prepare slide?

Such stains are called vital stains. For making temporary slides stains such as methylene blue, idodine, aniline hydrochloride, safranin etc are used.

Why is a stain used when preparing a slide?

Staining is done to visualise the specimen clearly under the microscope. Without staining the specimen under the microscope will look transparent or will not be visible clearly. Hence, staining is done to make the components of cell clearly visible.

What is the procedure for staining a slide?

Simple Stain Procedure Place your carefully prepared fixed smear slides on the stain rack over the sink. ... Let the stain sit for 1-5 minutes. Using the clothespin, grab the long end of the slide, tilt the slide over the sink and rinse the stain off with a stream of water from the wash bottle.

What do you stain slides with?

Microscope Slide Stains Iodine: Stains carbohydrates in plant and animal specimens brown or blue-black. ... Methylene blue: Stains acidic cell parts (like nucleus) blue. ... Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. ... Toluidene blue: Stains acidic cell parts (like nucleus) dark blue.

What is a stain used for in a microscope?

Microscope cell staining is a technique used to enable better visualization of cells and cell parts under the microscope. By using different stains, a nucleus or a cell wall are easier to view. Most stains can be used on non-living (fixed) cells, while only some types of stain can be used on living cells.

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Slide stain in DWD smoothly