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slide culture and lacto phenol cotton blue staining certain funky may be identified by observing Canadia for structure and spore production to prepare for this demonstration a block of several agar from a previously positively identified culture was aseptic lee cut and placed onto a fresh sub erode agar plate to grow a sterile cover slip was placed aseptically onto the transferred block of fungus my cilia and spores attached to this cover slip as the culture grows the plate was wrapped in parafilm and incubated at 26 degrees until the fungus had grown and spore elated staining the coverslip with lacto phenol cotton blue working inside a biological safety cabinet place a drop of lacto phenol cotton blue stain onto a clean glass slide remove the parafilm from the plate dispose into the appropriate waste container prise the coverslip gently from the agar block using a set of sterile tweezers place the coverslip by touching it to the stain at a 45-degree angle then carefully lowering it o
