Slide stain in cgi smoothly

Aug 6th, 2022
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How to slide stain in cgi with top efficiency

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How to Slide stain in cgi

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the funnest part of your CBC is making blood smears and its usually the bane of peoples existence but we are going to make it so you dont have any problems by the time this video is over first thing is you want to have a well-mixed unclouded EDTA tube obviously you want to mix it gently you dont want to be shaking it if youre concerned there may be clot in the sample which is obviously going to affect your numerical parameters most notably your platelets you can take an applicator stick and just run it through your sample and if there are any clots in there it will adhere to your applicator stick so if you dont have that you know you have a good sample the number one thing of making good smears is you want to make sure that you have bevel edge slides that is hugely important its almost impossible to make good blood smears without that as your tool and they can be used just for spreading if economy is an issue you want to start out with a well-filled PCV tube to get a decent siz

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0:00 0:56 How to Stain a Slide - YouTube YouTube Start of suggested clip End of suggested clip Were going to put a drop on one side. And it wicks over just a little bit. But if we take the paperMoreWere going to put a drop on one side. And it wicks over just a little bit. But if we take the paper towel and put it on the other side whoa. It basically sucks the stain across the slide.
Process Prepare a wet mount slide with a specimen. Place a single drop of stain on one outer edge of the cover slip on top of your slide. Place some paper towel against the opposite edge of the cover slip - as close to the edge as possible. The paper towel will draw the stain underneath the cover slip.
How to Stain Slides Prepare a wet mount or dry mount with a coverslip. Add a small drop of stain to an edge of the coverslip. Place the edge of a tissue or paper towel on the opposite edge of the coverslip. Capillary action will pull the dye across the slide to stain the specimen.
Staining is done to visualise the specimen clearly under the microscope. Without staining the specimen under the microscope will look transparent or will not be visible clearly. Hence, staining is done to make the components of cell clearly visible.
Microscope Slide Stains Iodine: Stains carbohydrates in plant and animal specimens brown or blue-black. Methylene blue: Stains acidic cell parts (like nucleus) blue. Eosin Y: Stains alkaline cell parts (like cytoplasm) pink. Toluidene blue: Stains acidic cell parts (like nucleus) dark blue.
In order to stain the bacterial specimen for microscopy one must first prepare the smear on the slide. This basically involves three steps----transferring a liquid suspension of the bacterium on the slide, drying the smear, and then heating slightly to firmly attach the smear to the slide.
Gram staining procedure Applying a primary stain (crystal violet). Adding a mordant (Grams iodine). Rapid decolorization with ethanol, acetone or a mixture of both. Counterstaining with safranin.
Trichrome stains (Masson) are used for distinguishing collagen from muscle tissue. In general, they consist of nuclear, collagenous and cytoplasmic dyes in mordants such as phosphotungstic or phosphomolybdic acid.
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
Simple Stain Procedure Place your carefully prepared fixed smear slides on the stain rack over the sink. Let the stain sit for 1-5 minutes. Using the clothespin, grab the long end of the slide, tilt the slide over the sink and rinse the stain off with a stream of water from the wash bottle.

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