Slide stain in binary smoothly

Aug 6th, 2022
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How to slide stain in binary with top efficiency

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How to Slide stain in binary

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so hi micro punter here I have a solution with the so called methylene blue according to Lafleur also known as the flood solution and in this video Im gonna show you how to use methylene blue to stain cells and specifically Im going to show you how to spay a stain the cheek cells Im also gonna take some cells from my teeth some bacteria that is Im gonna show you how you can stain your bacteria and at the very end Im going to put some drop of blood under the microscope and methylene blue is gonna stain the white blood cells but before I do that you have to be with me because Im going to now first tell you a little bit of background information some theory has to be when you buy yourself a set of stains and sometimes you will see that there are two bottles of methylene blue included one of them is called methylene blue just more saturated solution of methylene blue or concentrated methylene blue and then one is called methylene blue according to the flood or also known as basic me

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The process involves three steps: Cells are stained with crystal violet dye. A decolorizer such as ethyl alcohol or acetone is added to the sample, which dehydrates the peptidoglycan layer, shrinking and tightening it. A counterstain, such as the weakly water soluble safranin, is added to the sample, staining it red.
To view a Gram stain: Mount the slide on the stage with the smear facing the objective lens; if the slide is upside down, you wont be able to focus at high magnification. Rotate the condenser turret to the bright field position (O position). Move the aperture diaphragm knob to the center position (moderate contrast).
Staining is done to visualise the specimen clearly under the microscope. Without staining the specimen under the microscope will look transparent or will not be visible clearly. Hence, staining is done to make the components of cell clearly visible.
Why Stain Cells? The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
Biological Stains Acridine orange: It is a fluorescent cationic dye that is selective to nucleic acids. Coomassie blue: It is used in gel electrophoresis to stain the proteins blue. Crystal violet: It is used in Grams staining along with iodine to stain the bacterial cell wall in purple colour.
Based on the nature of chromogen, there are three types of stain. Acidic stain (Anionic stain) Basic stain (Cationic stain) neutral stain.
The Most Common Stains Used Haematoxylin and eosin combined make up a common stain most histology labs use first. No doubt youre familiar with at least haematoxylin, which is sometimes used alone. Combining this with eosin helps bring better definition to tissues and cells using a specific color chart.
Seven Types of Stain Oil Stain. Oil stains are the most widely available and the type of stain most people think of when they think of stain. Varnish Stain. Varnish stains resemble oil stains in every way but one. Gel Stain. Lacquer Stain. Water-Soluble Dye Stain. Metal-Complex (Metalized) Dye Stain.
Often used in clinical laboratories differential staining techniques include acid-fast staining, endospore staining and the very commonly used Gram staining.
A Gram stain is colored purple. When the stain combines with bacteria in a sample, the bacteria will either stay purple or turn pink or red. If the bacteria stays purple, they are Gram-positive. If the bacteria turns pink or red, they are Gram-negative.

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