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welcome back to our gcp mindset channel and to part two of our web seminar of sample size calculation in clinical trials in part two we talk about the role of the sample size calculations throughout all study phases in the second part we will have a look at the sample size and its role throughout the study phases and first of all what only already mentioned is that in clinical trials the different phases have very different objectives so its very uh important if youre on a phase one study or in a phase four study and the first thing we should notice is that the trial objective and the sample size justification must always match this is important the second thing is that when the the drug development proceed and we um yeah we obtain more evidence over the time then this evidence should always be included when we do a sample size calculation for example as you remember that when we um have to um make the sample size calculation we have to assume the treatment effect and the variance an