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Hello, Im Kristie Diebold, cleanroom manager and senior research microscopist at McCrone Associates, and Im also an instructor at Hooke College of Applied Sciences. A permanent sample preparation onto a glass slide can be made in a number of different ways: you can use immersion oils, you can also use different refractive index liquids, and also, a UV cured optical cement. Today I will be demonstrating the most common mounting medium, which is a thermoplastic resin with with the refractive index of 1.662. I have already begun heating a thermoplastic resin with the refractive index of 1.662. You want to heat the resin to a point where it is like hot maple syrup. Here I have a pre-cleaned glass slide, square cover glass, a microspatula, and a needle. Im going to be using these supplies to prep my sample. With my pre-cleaned glass slide already on a hot plate, Im going to add my sample. I am using a needle instead of a microspatula so that I can get a very small amount of sample on
