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[Music] we have completed tissue imaging now we will assemble the slide cassette we have our vision spatial gene expression slide with the fixed stain and image tissue we have a slide cassette and a gasket and the slide alignment tool insert the gasket and align the gasket and slide cassette cutouts ensure that the back of the slide cassette is facing the user prior to assembly align the label on top of the slide to the top of the slide cassette the active surface of the slide with tissue sections will be facedown such as the slide label is no longer readable insert the slide under tabs 1 and to ensure that the long edge of the slide is flush with the side of the slide cassette press the insert clip very firmly by applying even force on the lower part of the insert clip press down on the slide with a finger in between tabs three and four until the slide is under each tab and release the insert clip the slide cassette can also be assembled using the slide alignment tool position the sl
