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in this video im going to show you how to prepare a wet mount slide of stained onion epidermal cells heres my microscope slide i want to have that out and ready i can obtain my specimen from one layer of onion bulb if i look at this layer one side is kind of rough and matte and the other side is smooth and shiny i need to break my onion layer towards the shiny side and then very gently peel the two pieces apart theres a thin transparent layer of cells holding these two pieces of the onion layer together thats the layer of epidermal cells that im looking for for my specimen now i need to obtain a piece of that onion epidermis to place on my slide i can do that by tearing it off with a pair of tweezers this is a very delicate thin layer of cells and theyll fold over on themselves so when you place that onto the slide once you get it off your tweezers youre going to need to spread it out a little bit the little piece of onion epidermis can easily get all wadded up and that makes it
