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[Music] the following instructions are for the thermoscientific nunc lab tech 2 chamber slide system [Music] to begin aseptically peel back the tray cover if fewer than 8 slides are needed store the remaining slides in a sterile environment for example you can keep the remaining slides in the tray and seal the cover back on the tray with adhesive tape thermoscientific nunc lab tech 2 chamber slides are made with treated glass for optimal cell attachment the cc2 glass surface option is positively charged and suitable for culturing neurons using a serological pipet fill four chambers with 0.5 to 1 milliliters of medium and cell suspension in each well [Music] then replace the cover now its time to incubate the tray can serve as an incubation rack putting a damp paper towel in the bottom of the tray may minimize the evaporation from the wells once incubation is complete remove the slide from the tray and discard medium before staining is performed you can remove the chamber from the sli