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[Music] the following instructions are for the thermoscientific nunc lab tech chamber slide system [Music] to begin aseptically peel back the tray cover if fewer than eight slides are needed store the remaining slides in a sterile environment remove the chamber slide from the tray and set it inside a biological safety cabinet remove the media chamber cover using a serological pipette fill the chamber with point two to point four milliliters of medium and cell suspension into the wells of an eight weld chamber slide then replace the cover now its time to incubate the cells the tray can serve as an incubation rack putting a damp paper towel in the bottom of the tray can minimize the evaporation from the wells [Music] once incubation is complete take out the slide from the incubator and remove the medium from the wells [Music] to detach the slide from the media chamber grip the end of the slide with one hand gently squeeze both ends of the media chamber toward the center lifting the cham
