Slide epitaph in PAP smoothly

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Aug 6th, 2022
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How to Slide epitaph in PAP

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hi my name is Natalie Bonet Im an assistant professor of pathology and Laboratory Medicine I work at women infants hospital in Providence Rhode Island and Im recording a lecture today on gynecologic cytopathology this is a first in the series and today we will start at the beginning of the Bethesda book my contact information is noted on the slide here at the website and a Twitter handle please get in touch with me if you have any feedback today we will talk about report elements adequacy and non neoplastic findings and then at the end reporting of endometrial cells so just to start off the source for this lecture material was mostly the Bethesda system for reporting cervical cytology its a book that came out in 2015 the aim of this system is that the terminology must be number one clinically relevant number two reasonably reproducible and flexible and number three reflect the most current understanding of cervical neoplasia the bethesda system was convened for the first time in 198

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Atypical squamous cells of undetermined significance (ASC-US): This is the most common abnormal Pap test finding. It means that some cells dont look completely normal, but its not clear if the changes are caused by HPV infection.
Position Matters, Too. A simple change in positioning might also make a pap smear less painful. Some women tolerate the procedure better by lying on their sides or they place their hands underneath their rears and press down. Just like sex, different positions can decrease any discomfort you feel in your vagina.
In most cases, an abnormal Pap test is a result of: A human papillomavirus (HPV) infection. A sexually transmitted infection (STI or STD), such as herpes or trichomoniasis. A bacterial or yeast infection. Inflammation in the pelvic area. Normal cellular changes that occur with age.
Conventional Pap Smear: Cervical scrape and brushing is recommended. Except for evaluation of maturation index and detection of vaginal adenosis, submit only one slide. For routine Pap tests, both the scraping of the squamocolumnar junction and endocervical brushing should be spread onto the same slide.
Conventional Pap Smear: Cervical scrape and brushing is recommended. Except for evaluation of maturation index and detection of vaginal adenosis, submit only one slide. For routine Pap tests, both the scraping of the squamocolumnar junction and endocervical brushing should be spread onto the same slide.
Saline solution may be used for moistening. The lubricant does not dissolve during processing of the slide. It often stains dark purple and may hide numerous cells. Before scraping, the wooden spatula should be dipped in saline to moisten it and prevent too rapid dehydration of the scraped cells.
Rotate a spatula 360 once to obtain a single sample. Smear the sample onto the labeled slide. Fix the sample immediately (before it is air-dried) using a cytology spray fixative. Hold the fixative 15-20 cm (6 to 8 inches) away from the slide and evenly spray the slide by depressing the plunger 2 or 3 times.
Procedure Steps Smear test equipment. Examine the outer vagina. Insert the speculum. Advance the speculum. Place the tip of the cervical brush into the external cervical os. Place the end of the brush into the sample pot. Carefully remove the speculum from the vagina.

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