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hi my name is diane and today im going to show you how to create a slide for cytology evaluation from an aspirated sample whether its ultrasound guided or from a skin mass a lymph node or even a liquid sample such as a transtracheal wash abdominal fluid pericardial fusion or a urine sample basically anything you can obtain cells from the technique is pretty much the same no matter what your source is for your cells so always start with new slides if your sample was collected as a finite aspert from a mass your entire sample will be in this needle very likely so what you need to do is remove the syringe draw back some air reattach position your needle over your slide spray your sample onto the slide repeat this because you may have more sample in there and you dont want to waste any of it spray it again and if you think you have more sample try it again because like i said you want to have as many slides as you can to smear this you take a new clean slide take one of your samples lay