Slide attachment in the protocol

Aug 6th, 2022
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How to slide attachment in the protocol

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Welcome to this Precision Labs presentation on ethernet. In this session, well spend a few minutes looking at ethernet physical layer transceivers, also called PHYs. In this video, well cover the following topics. How a PHY is connected in a typical application circuit, a breakdown of the PHY into common sub-functions, and the capabilities of each sub-function. This diagram shows a basic system level implementation of an ethernet physical layer connection. The PHY sits between the media access controller device, or MAC, and the network connection. The MAC device can be either a microcontroller, processor, FPGA, or ethernet switch. The connection to the MAC layer is called the media independent interface, or MII. Different versions of this interface are defined in the IEEE 802.3 standard and offer either reduced number of signal lines, higher data speeds, or both. The supported data speeds are 10, 100, 1,000 megabits per second and higher. The term XMII is used to collectively refer t

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Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.
PLL is often used as a coating agent to promote cell adhesion in culture. PLL surface treatments improve the survival of many primary cells in culture and support neurite outgrowth. This PLL solution is provided as a stock solution (10 mg/ml) and contains polymers in the 70,000 - 150,000 kDa range.
Poly-L-lysine is a nonspecific attachment factor for cells and is useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
-poly-L-lysine (-PL) is a naturally occurring poly(amino acid) of varying polymerization degree, which possesses excellent antimicrobial activity and has been widely used in food and pharmaceutical industries.
Poly-D-Lysine promotes the adhesion of cultured cell onto coverslips. The polycationic property of the molecule allows interaction with anionic sites on the cells promoting effective attachment to the growing surface.
The principle behind the method is that the polycationic polylysine molecules adsorb strongly to various solid surfaces, leaving cationic sites which combine with the anionic sites on cell surfaces.
Poly-lysine is a synthetic positively charged polymer, existing as two enantiomers, poly-D-lysine (PDL) and poly-L-lysine (PLL). Both are commonly used however poly-D-lysine is not degraded by cellular proteases and is therefore often the preferred choice.
Micropatterning in Cell Biology Part A It consists of a poly(l-lysine) backbone with grafted poly(ethylene glycol) side chains. The PLL is positively charged and therefore interacts with negatively charged surfaces.

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