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bring all the reagents at room temperature label the glass slide with ID place the semen on the glass slide and prepare a smear keep the slide on the slide warmer to dry dip the slide in fixative 95 percent ethanol keep it for 15 minutes drain of the solution keep the slide on the slide warmer to dry keep the slide horizontally on a staining tray cover the smell with 70% ethanol keep it for 30 seconds cover the smell with 50 percent ethanol keep it for 30 seconds dip the slide in distilled water and keep it for 30 seconds drain off the water keep the slide horizontally on a staining tray cover the smear with harriss hematoxylin keep it for four minutes drain off the stain dip the slide in distilled water and keep it for 30 seconds drain off the distilled water keep the slide horizontally on a staining tray cover the smear with acid ethanol keep it for 10 seconds hole the smear under running water for 5 minutes [Music] drain off the water keep the slide horizontally on a staining tray